At least 25,000 people protested in Pakistan's largest demonstration yet against an anti-Quran film made by a Dutch lawmaker, urging their government to expel the ambassadors of the Netherlands and Denmark.

The 15-minute film by Geert Wilders, which sets verses from the Muslim holy book against a background of violent images from terror attacks, was released in March. It has prompted weekly protests in Pakistan, usually drawing hundreds of people after prayers on Friday, the Muslim holy day.

Police officer Syed Suleman estimated Sunday's crowd at 25,000, while organizers claimed more than 100,000 people turned out.

"They call this freedom of expression, but it's freedom of aggression," Munawwar Hasan, a leader of the main Islamic party Jamaat-e-Islami, told the crowd.

Wearing head bands inscribed "We are ready to sacrifice our lives for the sanctity of the prophet," protesters marched for more than a mile, then gathered on Karachi's main street to listen to speeches. They also burned an effigy of Wilders.

"The Muslim world is on one side, but Muslim rulers like (President Pervez) Musharraf are toeing the Western agenda under the garb of secularism," Hasan said, referring to Musharraf's role in Washington's war on terrorism.

Another Jamaat-e-Islami leader called for expulsion of the ambassadors of the Netherlands and Denmark, where newspapers have reprinted drawings considered blasphemous to Prophet Muhammad.

"We demand of the government to expel the Holland and Denmark diplomats from the country," said Mohammed Hussain Mehanati.

ASHRAF KHAN